This proposal addresses (1) the Fiscal Year 2016 (FY16) Peer Reviewed Cancer Research Program Topic Areas of Listeria vaccine for cancer, colorectal cancer, and immunotherapy and (2) FY16 Military Relevance Focus Area gaps in cancer prevention, screening, early detection, diagnosis, treatment, and/or survivorship that ... affect the general population but have a particularly profound impact on the health and well-being of military members, Veterans, and their beneficiaries. The objective of this proposal is to define the ability of a new vaccine to treat colorectal cancer in animal models. The vaccine will use a crippled version of Listeria monocytogenes to deliver a colorectal cancer protein to immune cells within the body to educate them against colorectal cancer. These educated immune cells may then be able to search for and kill colorectal cancer hiding in the body. If successful, the results of these studies could be applied to patients with metastatic colorectal cancer, a disease that is typically fatal. This would require several years of work to test the vaccine in animal models before testing in humans. Beyond colorectal cancer, this new vaccine also may be effective against other common forms of cancer, including stomach, esophageal, and pancreatic cancers, which require new, more effective treatments. Importantly, about half of military personnel and dependents diagnosed with colorectal cancer die of the disease, highlighting the potential impact this research identifying a new colorectal cancer treatment could have on the lives of Service members, their families, and the general population.